Identification of client iron-sulfur proteins of the chloroplastic NFU2 transfer protein in Arabidopsis thaliana.
Identifieur interne : 000107 ( Main/Exploration ); précédent : 000106; suivant : 000108Identification of client iron-sulfur proteins of the chloroplastic NFU2 transfer protein in Arabidopsis thaliana.
Auteurs : Nathalie Berger [France] ; Florence Vignols [France] ; Jonathan Przybyla-Toscano [France] ; Mélanie Roland [France] ; Valérie Rofidal [France] ; Brigitte Touraine [France] ; Krzysztof Zienkiewicz [Allemagne] ; Jérémy Couturier [France] ; Ivo Feussner [Allemagne] ; Véronique Santoni [France] ; Nicolas Rouhier [France] ; Frédéric Gaymard [France] ; Christian Dubos [France]Source :
- Journal of experimental botany [ 1460-2431 ] ; 2020.
Abstract
Iron-sulfur (Fe-S) proteins have critical functions in plastids, notably participating in photosynthetic electron transfer, sulfur and nitrogen assimilation, chlorophyll metabolism, and vitamin or amino acid biosynthesis. Their maturation relies on the so-called SUF (sulfur mobilization) assembly machinery. Fe-S clusters are synthesized de novo on a scaffold protein complex and then delivered to client proteins via several transfer proteins. However, the maturation pathways of most client proteins and their specificities for transfer proteins are mostly unknown. In order to decipher the proteins interacting with the Fe-S cluster transfer protein NFU2, one of the three plastidial representatives found in Arabidopsis thaliana, we performed a quantitative proteomic analysis of shoots, roots, and seedlings of nfu2 plants, combined with NFU2 co-immunoprecipitation and binary yeast two-hybrid experiments. We identified 14 new targets, among which nine were validated in planta using a binary bimolecular fluorescence complementation assay. These analyses also revealed a possible role for NFU2 in the plant response to desiccation. Altogether, this study better delineates the maturation pathways of many chloroplast Fe-S proteins, considerably extending the number of NFU2 clients. It also helps to clarify the respective roles of the three NFU paralogs NFU1, NFU2, and NFU3.
DOI: 10.1093/jxb/eraa166
PubMed: 32240305
Affiliations:
- Allemagne, France
- Basse-Saxe, Grand Est, Languedoc-Roussillon, Lorraine (région), Occitanie (région administrative)
- Göttingen, Montpellier, Nancy
- Université de Lorraine
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last="Couturier">Jérémy Couturier</name><affiliation wicri:level="4"><nlm:affiliation>Université de Lorraine, INRAE, IAM, Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, INRAE, IAM, Nancy</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Feussner, Ivo" sort="Feussner, Ivo" uniqKey="Feussner I" first="Ivo" last="Feussner">Ivo Feussner</name><affiliation wicri:level="3"><nlm:affiliation>Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Service unit for Metabolomics and Lipidomics, Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Service unit for Metabolomics and Lipidomics, Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation></author><author><name sortKey="Santoni, Veronique" sort="Santoni, Veronique" uniqKey="Santoni V" first="Véronique" last="Santoni">Véronique 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type="wicri:Area/Main/Exploration">000116</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of client iron-sulfur proteins of the chloroplastic NFU2 transfer protein in Arabidopsis thaliana.</title><author><name sortKey="Berger, Nathalie" sort="Berger, Nathalie" uniqKey="Berger N" first="Nathalie" last="Berger">Nathalie Berger</name><affiliation wicri:level="3"><nlm:affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Vignols, Florence" sort="Vignols, Florence" uniqKey="Vignols F" first="Florence" last="Vignols">Florence Vignols</name><affiliation wicri:level="3"><nlm:affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Przybyla Toscano, Jonathan" sort="Przybyla Toscano, Jonathan" uniqKey="Przybyla Toscano J" first="Jonathan" last="Przybyla-Toscano">Jonathan Przybyla-Toscano</name><affiliation wicri:level="4"><nlm:affiliation>Université de Lorraine, INRAE, IAM, Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, INRAE, IAM, Nancy</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Roland, Melanie" sort="Roland, Melanie" uniqKey="Roland M" first="Mélanie" last="Roland">Mélanie Roland</name><affiliation wicri:level="4"><nlm:affiliation>Université de Lorraine, INRAE, IAM, Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, INRAE, IAM, Nancy</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Rofidal, Valerie" sort="Rofidal, Valerie" uniqKey="Rofidal V" first="Valérie" last="Rofidal">Valérie Rofidal</name><affiliation wicri:level="3"><nlm:affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Touraine, Brigitte" sort="Touraine, Brigitte" uniqKey="Touraine B" first="Brigitte" last="Touraine">Brigitte Touraine</name><affiliation wicri:level="3"><nlm:affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Zienkiewicz, Krzysztof" sort="Zienkiewicz, Krzysztof" uniqKey="Zienkiewicz K" first="Krzysztof" last="Zienkiewicz">Krzysztof Zienkiewicz</name><affiliation wicri:level="3"><nlm:affiliation>Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation></author><author><name sortKey="Couturier, Jeremy" sort="Couturier, Jeremy" uniqKey="Couturier J" first="Jérémy" last="Couturier">Jérémy Couturier</name><affiliation wicri:level="4"><nlm:affiliation>Université de Lorraine, INRAE, IAM, Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, INRAE, IAM, Nancy</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Feussner, Ivo" sort="Feussner, Ivo" uniqKey="Feussner I" first="Ivo" last="Feussner">Ivo Feussner</name><affiliation wicri:level="3"><nlm:affiliation>Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Service unit for Metabolomics and Lipidomics, Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Service unit for Metabolomics and Lipidomics, Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Göttingen</settlement></placeName></affiliation></author><author><name sortKey="Santoni, Veronique" sort="Santoni, Veronique" uniqKey="Santoni V" first="Véronique" last="Santoni">Véronique Santoni</name><affiliation wicri:level="3"><nlm:affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Rouhier, Nicolas" sort="Rouhier, Nicolas" uniqKey="Rouhier N" first="Nicolas" last="Rouhier">Nicolas Rouhier</name><affiliation wicri:level="4"><nlm:affiliation>Université de Lorraine, INRAE, IAM, Nancy, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Lorraine, INRAE, IAM, Nancy</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Lorraine (région)</region><settlement type="city">Nancy</settlement></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Gaymard, Frederic" sort="Gaymard, Frederic" uniqKey="Gaymard F" first="Frédéric" last="Gaymard">Frédéric Gaymard</name><affiliation wicri:level="3"><nlm:affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Dubos, Christian" sort="Dubos, Christian" uniqKey="Dubos C" first="Christian" last="Dubos">Christian Dubos</name><affiliation wicri:level="3"><nlm:affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Iron-sulfur (Fe-S) proteins have critical functions in plastids, notably participating in photosynthetic electron transfer, sulfur and nitrogen assimilation, chlorophyll metabolism, and vitamin or amino acid biosynthesis. Their maturation relies on the so-called SUF (sulfur mobilization) assembly machinery. Fe-S clusters are synthesized de novo on a scaffold protein complex and then delivered to client proteins via several transfer proteins. However, the maturation pathways of most client proteins and their specificities for transfer proteins are mostly unknown. In order to decipher the proteins interacting with the Fe-S cluster transfer protein NFU2, one of the three plastidial representatives found in Arabidopsis thaliana, we performed a quantitative proteomic analysis of shoots, roots, and seedlings of nfu2 plants, combined with NFU2 co-immunoprecipitation and binary yeast two-hybrid experiments. We identified 14 new targets, among which nine were validated in planta using a binary bimolecular fluorescence complementation assay. These analyses also revealed a possible role for NFU2 in the plant response to desiccation. Altogether, this study better delineates the maturation pathways of many chloroplast Fe-S proteins, considerably extending the number of NFU2 clients. It also helps to clarify the respective roles of the three NFU paralogs NFU1, NFU2, and NFU3.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">32240305</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>71</Volume><Issue>14</Issue><PubDate><Year>2020</Year><Month>Jul</Month><Day>06</Day></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>Identification of client iron-sulfur proteins of the chloroplastic NFU2 transfer protein in Arabidopsis thaliana.</ArticleTitle><Pagination><MedlinePgn>4171-4187</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/eraa166</ELocationID><Abstract><AbstractText>Iron-sulfur (Fe-S) proteins have critical functions in plastids, notably participating in photosynthetic electron transfer, sulfur and nitrogen assimilation, chlorophyll metabolism, and vitamin or amino acid biosynthesis. Their maturation relies on the so-called SUF (sulfur mobilization) assembly machinery. Fe-S clusters are synthesized de novo on a scaffold protein complex and then delivered to client proteins via several transfer proteins. However, the maturation pathways of most client proteins and their specificities for transfer proteins are mostly unknown. In order to decipher the proteins interacting with the Fe-S cluster transfer protein NFU2, one of the three plastidial representatives found in Arabidopsis thaliana, we performed a quantitative proteomic analysis of shoots, roots, and seedlings of nfu2 plants, combined with NFU2 co-immunoprecipitation and binary yeast two-hybrid experiments. We identified 14 new targets, among which nine were validated in planta using a binary bimolecular fluorescence complementation assay. These analyses also revealed a possible role for NFU2 in the plant response to desiccation. Altogether, this study better delineates the maturation pathways of many chloroplast Fe-S proteins, considerably extending the number of NFU2 clients. It also helps to clarify the respective roles of the three NFU paralogs NFU1, NFU2, and NFU3.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Berger</LastName><ForeName>Nathalie</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vignols</LastName><ForeName>Florence</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Przybyla-Toscano</LastName><ForeName>Jonathan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Université de Lorraine, INRAE, IAM, Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Roland</LastName><ForeName>Mélanie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Université de Lorraine, INRAE, IAM, Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rofidal</LastName><ForeName>Valérie</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Touraine</LastName><ForeName>Brigitte</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zienkiewicz</LastName><ForeName>Krzysztof</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Couturier</LastName><ForeName>Jérémy</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Université de Lorraine, INRAE, IAM, Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Feussner</LastName><ForeName>Ivo</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Service unit for Metabolomics and Lipidomics, Göttingen Center for Molecular Biosciences (GZMB), University of Göttingen, Göttingen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Santoni</LastName><ForeName>Véronique</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rouhier</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Université de Lorraine, INRAE, IAM, Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gaymard</LastName><ForeName>Frédéric</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dubos</LastName><ForeName>Christian</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>BPMP, Université de Montpellier, CNRS, INRAE, SupAgro, Montpellier, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arabidopsis</Keyword><Keyword MajorTopicYN="N">NFU2</Keyword><Keyword MajorTopicYN="N">chloroplast</Keyword><Keyword MajorTopicYN="N">iron–sulfur cluster</Keyword><Keyword MajorTopicYN="N">protein–protein interactions</Keyword><Keyword MajorTopicYN="N">quantitative proteomic analysis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>01</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32240305</ArticleId><ArticleId IdType="pii">5815136</ArticleId><ArticleId IdType="doi">10.1093/jxb/eraa166</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>France</li></country><region><li>Basse-Saxe</li><li>Grand Est</li><li>Languedoc-Roussillon</li><li>Lorraine (région)</li><li>Occitanie (région administrative)</li></region><settlement><li>Göttingen</li><li>Montpellier</li><li>Nancy</li></settlement><orgName><li>Université de Lorraine</li></orgName></list><tree><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Berger, Nathalie" sort="Berger, Nathalie" uniqKey="Berger N" first="Nathalie" last="Berger">Nathalie Berger</name></region><name sortKey="Couturier, Jeremy" sort="Couturier, Jeremy" uniqKey="Couturier J" first="Jérémy" last="Couturier">Jérémy Couturier</name><name sortKey="Dubos, Christian" sort="Dubos, Christian" uniqKey="Dubos C" first="Christian" last="Dubos">Christian Dubos</name><name sortKey="Gaymard, Frederic" sort="Gaymard, Frederic" uniqKey="Gaymard F" first="Frédéric" last="Gaymard">Frédéric Gaymard</name><name sortKey="Przybyla Toscano, Jonathan" sort="Przybyla Toscano, Jonathan" uniqKey="Przybyla Toscano J" first="Jonathan" last="Przybyla-Toscano">Jonathan Przybyla-Toscano</name><name sortKey="Rofidal, Valerie" sort="Rofidal, Valerie" uniqKey="Rofidal V" first="Valérie" last="Rofidal">Valérie Rofidal</name><name sortKey="Roland, Melanie" sort="Roland, Melanie" uniqKey="Roland M" first="Mélanie" last="Roland">Mélanie Roland</name><name sortKey="Rouhier, Nicolas" sort="Rouhier, Nicolas" uniqKey="Rouhier N" first="Nicolas" last="Rouhier">Nicolas Rouhier</name><name sortKey="Santoni, Veronique" sort="Santoni, Veronique" uniqKey="Santoni V" first="Véronique" last="Santoni">Véronique Santoni</name><name sortKey="Touraine, Brigitte" sort="Touraine, Brigitte" uniqKey="Touraine B" first="Brigitte" last="Touraine">Brigitte Touraine</name><name sortKey="Vignols, Florence" sort="Vignols, Florence" uniqKey="Vignols F" first="Florence" last="Vignols">Florence Vignols</name></country><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Zienkiewicz, Krzysztof" sort="Zienkiewicz, Krzysztof" uniqKey="Zienkiewicz K" first="Krzysztof" last="Zienkiewicz">Krzysztof Zienkiewicz</name></region><name sortKey="Feussner, Ivo" sort="Feussner, Ivo" uniqKey="Feussner I" first="Ivo" last="Feussner">Ivo Feussner</name><name sortKey="Feussner, Ivo" sort="Feussner, Ivo" uniqKey="Feussner I" first="Ivo" last="Feussner">Ivo Feussner</name></country></tree></affiliations></record>Pour manipuler ce document 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